RNA

Part:BBa_M1141:Design

Designed by: Micah Parrish   Group: UtahState_BE5930_-_S11   (2011-04-24)

PHALNPOX Phanerochaete chrysosporium lignin peroxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 603
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 792
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 360
    Illegal BsaI site found at 528
    Illegal BsaI site found at 1204
    Illegal BsaI.rc site found at 1059
    Illegal SapI site found at 35


Design Notes

In this original sequence pstI cut at 644 at sequence CTGCAG and it was necessary to get rid of that pstI restriction site so CTG (leucine) was changed to CTA (also leucine) to get rid of the pstI restriction site.

Source

Phanerochaete chrysosporium is from a white rot fungi

References